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R0280

Sigma-Aldrich

Anti-RanGAP1 (N-terminal) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution, ~2 mg/mL

Synonym(s):

Anti-Ran-GTPase-Activating Protein 1

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About This Item

MDL number:
MFCD09265372
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~2 mg/mL

technique(s)

indirect immunofluorescence: 10-20 μg/mL using HeLa cells
western blot: 1-2 μg/mL using HEK-293T cells transfected with human RanGAP1

UniProt accession no.

P46060

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RANGAP1(5905)
mouse ... Rangap1(19387)

Related Categories

General description

Ran GTPase−activating protein 1 (RanGAP1), a guanosine-5′-triphosphatase (GTPase)-activating protein for Ran exists in two different types, where one is cytoplasmic and other is concerted at the cytoplasmic fibers of nuclear pore complexes (NPCs). Mammalian RanGAP1 has a C-terminal extension. Ran GTPase gene is located on human chromosome 22q13.2.

Specificity

Anti-RanGAP1 (N-terminal) recognizes human RanGAP1.

Immunogen

synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of human RanGAP1, conjugated to KLH. This sequence is identical in mouse RanGAP1.

Application

Anti-RanGAP1 (N-terminal) antibody produced in rabbit may be used immunoblotting and immunofluorescence.

Biochem/physiol Actions

Ran GTPase−activating protein 1 (RanGAP1) regulates Ran activity. RanGAP1 can stimulate the GTPase activity of Ran. Ran is also regulated by a chromatin-bound nucleotide exchange factor, regulator of chromosome condensation 1 (RCC1) that keeps Ran in the active guanosine-5′-triphosphate (GTP)-bound state. RanGAP1 has the ability to conjugate to the small ubiquitin-related modifier protein small ubiquitin-related modifier 1 (SUMO-1). RanGAP1 may participate in nuclear protein import.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex
Matunis MJ, et al.
The Journal of cell biology, 140(3), 499-509 (1998)
A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
Mahajan R, et al.
Cell, 88(1), 97-107 (1997)
Circular RNA circ-RanGAP1 regulates VEGFA expression by targeting miR-877-3p to facilitate gastric cancer invasion and metastasis
Lu J, et al.
Cancer Letters, 471, 38-48 (2020)
Ubc9p and the conjugation of SUMO-1 to RanGAP1 and RanBP2
Saitoh H, et al.
Current Biology, 8(2), 121-124 (1998)

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