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R0525

Sigma-Aldrich

RANK Ligand from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

Osteoclast differentiation factor (ODF), Osteoprotegerin ligand (OPGL), Receptor activator of NF-κB Ligand (RANKL), TNF-related activation−induced cytokines (TRANCE)

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About This Item

MDL number:
MFCD01867142
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

Quality Level

200

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

10.0-25.0 ng/mL ED50

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/mg
<1 EU/μg

color

white

UniProt accession no.

O35235

storage temp.

−20°C

Gene Information

mouse ... Tnfsf11(21943)

Related Categories

General description

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or receptor activator of nuclear factor-κB ligand (RANKL) is a type II transmembrane signaling receptor. It has a molecular weight of about 35kDa. The protein possesses an amino-terminal intracellular tail and a carboxy-terminal extracellular region, which has a connecting stalk and a receptor-binding domain.

Application

RANK ligand from mouse has been used to visualize differentiated osteoclasts and to stimulate osteoclast differentiation.
The functions of RANK Ligand include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function induction of osteoclastogenesis, and lymph node organogenesis.The cell surface signaling receptor of RANK Ligand is RANK, which undergoes receptor clustering during signal transduction.

Biochem/physiol Actions

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or RANKL has an important role in osteoclast function and differentiation. It binds to RANK and osteoprotegrin (OPG). Bioactivities of RANKL may be attributed to its binding to these two receptor molecules. Mouse and human RANKL share 85% amino acid sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution of 5 mM sodium phosphate, pH 7.6, and 75 mM NaCl.

Analysis Note

The bioactivity is determined by its ability to induce osteoclast differentiation of RAW 264.7. The expected ED50 for this effect is ≤10 ng/ml.

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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