R5404
Monoclonal Anti-Rab9 antibody produced in mouse
~2 mg/mL, clone Rab9-29, purified immunoglobulin, buffered aqueous solution
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Rab9-29, monoclonal
form
buffered aqueous solution
mol wt
~23 kDa
species reactivity
human, rat, hamster, canine, mouse
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2 μg/mL using total cell extract of HeLa cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAB9A(9367)
mouse ... Rab9(56382)
rat ... Rab9a(84589)
General description
Monoclonal Anti-Rab9 (mouse IgG1 isotype) is derived from the hybridoma Rab9-29 produced by the fusion of mouse cells (NS1 cells) and splenocytes from BALB/c mice. Ras-related protein Rab-9A (Rab9) is a member of the Rab family of small guanosine triphosphatases (GTPases). The gene Rab9 is mapped to human chromosome Xp22.2. Rab9 GTPase is localized predominantly to late endosomes.
Specificity
Monoclonal Anti-Rab9 recognizes human, canine, rat, mouse and hamster Rab9 (approx. 23 kDa)
Immunogen
synthetic peptide corresponding to amino acids 187-200 of human Rab9, conjugated to KLH.
Application
Monoclonal Anti-Rab9 antibody produced in mouse has been used in:
- western blotting
- enzyme-linked immunosorbent assay(ELISA)
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
Ras-related protein Rab-9A (Rab9) guanosine triphosphatase (GTPase) is required for the transport of mannose 6-phosphate receptors (MRPs) from endosomes to the trans-Golgi network (TGN). Rab GTPases are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Rab9 GTPase in its GTP bound conformation binds specifically to an effector protein named p40 that stimulates the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the TGN. Rab9 also interacts with the vesicle cargo selection protein tail-interacting protein of 47 kDa (TIP47). This interaction is required for Rab9 stability on late endosomes and may be crucial for vesicular transport in the virus life cycle.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Daniel P Kloer et al.
The Journal of biological chemistry, 285(10), 7794-7804 (2010-01-06)
The Hermansky-Pudlak syndrome (HPS) is a genetic hypopigmentation and bleeding disorder caused by defective biogenesis of lysosome-related organelles (LROs) such as melanosomes and platelet dense bodies. HPS arises from mutations in any of 8 genes in humans and 16 genes
Daniela Ploen et al.
European journal of cell biology, 92(12), 374-382 (2014-02-01)
Hepatitis C virus (HCV) morphogenesis and release are closely linked to lipid metabolism. It has been described recently by our group that TIP47 plays an essential role for the targeting of the NS5A-complexed RNA genome from the replicon complex to
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Communicative & integrative biology, 9(4), e1204498-e1204498 (2016-08-31)
The small GTPase Rab9 has long been described as a protein that mediates endosome-to-trans-Golgi Network (TGN) transport, and specifically mannose-6-phospate receptor (MPR) recycling. However, studies have challenged this view by showing that Rab9 also is connected to sorting pathways toward
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Salmonella's success at proliferating intracellularly and causing disease depends on the translocation of a major virulence protein, SifA, into the host cell. SifA recruits membranes enriched in lysosome associated membrane protein 1 (LAMP1) and is needed for growth of Salmonella
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