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R5279

Sigma-Aldrich

Anti-Rab9 from rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Ras-Associated Protein Rab9, Anti-Ras-Related Protein Rab9

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About This Item

MDL number:
MFCD03097193
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~23 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH3T3 cells fixed and permeabilized with 3% paraformaldehyde followed by 0.4% saponin
western blot: 5-10 μg/mL using whole extract of human A431 cells

UniProt accession no.

P51151

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB9A(9367)
mouse ... Rab9(56382)
rat ... Rab9a(84589)

General description

Rab9 is a member of the Rab family of guanosine triphosphatases (GTPases). Rab9 GTPase is localized predominantly to late endosomes. Rab proteins contain conserved regions involved in guanine nucleotide binding, and hypervariable COOH-terminal domains with a cysteine motif implicated in subcellular targeting.

Immunogen

synthetic peptide corresponding to amino acid residues 188-200 of human Rab9, conjugated to KLH.

Application

Anti-Rab9 from rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the trans-Golgi network (TGN). Rab9 GTPase in its GTP bound conformation binds specifically to an effector protein named p40 that stimulates the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the TGN. Rab9 also interacts with the vesicle cargo selection protein TIP47 (tail-interacting protein of 47 kDa). This interaction is required for Rab9 stability on late endosomes. Anti-Rab9 antibodies may be used as a marker for late endosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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High Resolution Crystal Structure of Human Rab9 GTPase a Novel Antiviral Drug Target
Chen L, et al.
The Journal of Biological Chemistry, 279(38), 40204-40208 (2004)
Rab9 GTPase regulates late endosome size and requires effector interaction for its stability
Ganley IG, et al.
Molecular Biology of the Cell, 15(12), 5420-5430 (2004)
Liqing Chen et al.
The Journal of biological chemistry, 279(38), 40204-40208 (2004-07-21)
Rab GTPases and their effectors facilitate vesicular transport by tethering donor vesicles to their respective target membranes. Rab9 mediates late endosome to trans-Golgi transport and has recently been found to be a key cellular component for human immunodeficiency virus-1, Ebola
The rab gtpase family
Stenmark H and Olkkonen VM
Genome Biology, 2(5), 3007-3001 (2001)
Small GTP-binding proteins
Takai Y, et al.
Physiological Reviews, 81(1), 153-208 (2001)
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