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RAB0200

Sigma-Aldrich

Gastrin EIA Kit

for serum, plasma, culture supernatant and cell lysates

Synonym(s):

Gastrin

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

species reactivity

human, mouse, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
enzyme immunoassay: suitable

input

sample type serum
sample type plasma
sample type culture supernatant(s)
sample type cell lysate

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 9.92 pg/mL
standard curve range: 0.1–1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GAST(2520)

Related Categories

General description

The Gastrin-I Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Gastrin-I peptide based on the principle of Competitive Enzyme Immunoassay.
Gastrin is a peptide hormone released in gastrointestinal region of the body. It is specifically produced by the G-cells in stomach and duodenum. During embryogenesis, gastrin secretion is observed only in the pancreas. Gastrin is also localized to the renal tubules. The GAST gene is mapped to human chromosome 17q21.2.

Immunogen

Gastrin, synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Gastrin EIA Kit has been used to estimate the levels of gastrin hormone in plasma or serum sample.

Biochem/physiol Actions

Gastrin is responsible for epithelial cell proliferation and differentiation. Gastrin regulates the action of parietal cells in secretion of gastric acid and growth factors essential for stomach maintenance. Gastrin maintains sodium ion homeostasis and controls body′s blood pressure. Hypoxial conditions in in vitro and in vivo analysis may elevate the expression of gastrin hormone in cancer cells in order to promote angiogenesis.

Kit Components Also Available Separately

Product No.
Description
SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

Certificates of Analysis (COA)

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Genomic analysis reveals the molecular heterogeneity of ovarian clear cell carcinomas.
Tan D S, et al.
Clinical Cancer Research, 17(6), 1521-1534 (2011)
The gastrin-releasing peptide analog bombesin preserves exocrine and endocrine pancreas morphology and function during parenteral nutrition.
Pierre J F, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 309(6), G431-G442 (2015)
Increased gastrin gene expression provides a physiological advantage to mice under hypoxic conditions.
Laval M, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 308(2), G76-G84 (2014)
Pancreatic beta cells express the fetal islet hormone gastrin in rodent and human diabetes.
Dahan T, et al.
Diabetes (2016)
Gastrin induces parathyroid hormone-like hormone expression in gastric parietal cells.
Al Menhali A, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 312(6), G649-G657 (2017)
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