Skip to Content
MilliporeSigma
All Photos(2)

Documents

SAB4600176

Sigma-Aldrich

Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody produced in donkey

~2 mg/mL, affinity isolated antibody

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

donkey

Quality Level

conjugate

CF 647 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

~2 mg/mL

technique(s)

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect immunofluorescence: 1-10 μg/mL

fluorescence

λex 650 nm; λem 665 nm

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG) is a glycoprotein antibody with two equivalent light chains and a pair of identical heavy chains. CF 647 is an organic cyanine dye useful in fluorescent microscopy studies. Antibody–CF 647 conjugates are affinity-purified antibodies labeled with a far-red fluorescent dye, CF 647. CF dyes are excellent alternatives to Alexa Fluor dyes for antibody labeling with exceptional brightness, photostability, and/or specificity.

Specificity

Binds all mouse IgGs (minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit and sheep serum proteins)

Immunogen

mouse IgG (H+L)

Biochem/physiol Actions

Immunoglobulin G (IgG) on pepsin digestion results in fragment crystallizable (Fc), which comprises the H chain constant region. Papain digestion of IgG results in the generation of fragment antigen-binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.

Preparation Note

Protect from light.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Blue-conversion of Organic Dyes Produces Artifacts in Multicolor Fluorescence Imaging
Kim DH, et al.
Chemical Science null
Harry W Schroeder et al.
The Journal of allergy and clinical immunology, 125(2 Suppl 2), S41-S52 (2010-03-05)
Immunoglobulins are heterodimeric proteins composed of 2 heavy and 2 light chains. They can be separated functionally into variable domains that bind antigens and constant domains that specify effector functions, such as activation of complement or binding to Fc receptors.
Gestur Vidarsson et al.
Frontiers in immunology, 5, 520-520 (2014-11-05)
Of the five immunoglobulin isotypes, immunoglobulin G (IgG) is most abundant in human serum. The four subclasses, IgG1, IgG2, IgG3, and IgG4, which are highly conserved, differ in their constant region, particularly in their hinges and upper CH2 domains. These

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service