Skip to Content
MilliporeSigma
All Photos(1)

Documents

SRE0053

Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

Suitable for manufacturing of diagnostic kits and reagents., lyophilized powder

Synonym(s):

Glycyl-glycine Endopeptidase

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9011-93-2
Enzyme Commission number:
3.4.24.75 (BRENDA, IUBMB)
MDL number:
MFCD00131556
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

bacterial (Staphylococcus sp.)

Quality Level

400

form

lyophilized powder

specific activity

≥500 units/mg protein

mol wt

26,926 Da

composition

protein, 50-70% biuret

packaging

pkg of (package size sold on basis of mg protein)

concentration

50—70% protein

suitability

suitable for microbiological tests

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Lysostaphin is a glycylglycine endopeptidase. It is an important antibacterial enzyme.

Biochem/physiol Actions

Lysostaphin has the ability to cleave both viable and heat-killed S. aureus cells. It is one of the major antibacterial agents for the staphylococci. Lysostaphin cleaves the cross-linking pentaglycine bridges in the cell walls of staphylococci. Thus it is considered as an antistaphylococcal therapeutic agent.
Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH7.5 at 37°C in a 6.0ml reaction mixture.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 6

1 of 6

Mutanolysin from Streptomyces globisporus ATCC 21553 0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret)

Sigma-Aldrich

M4782

Mutanolysin from Streptomyces globisporus ATCC 21553

Lysozyme from chicken egg white BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein

Sigma-Aldrich

L4919

Lysozyme from chicken egg white

Lyticase from Arthrobacter luteus lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

Sigma-Aldrich

L2524

Lyticase from Arthrobacter luteus

Chitinase from Streptomyces griseus lyophilized powder (essentially salt free), ≥200 units/g solid

Sigma-Aldrich

C6137

Chitinase from Streptomyces griseus

Lysozyme from chicken egg white lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Sigma-Aldrich

L6876

Lysozyme from chicken egg white

Lysozyme from hen egg white

Roche

10837059001

Lysozyme

Lysostaphin: a new bacteriolytic agent for the Staphylococcus
Schindler, Ch A and Schuhardt, VT
Proceedings of the National Academy of Sciences of the USA, 51(3), 414-421 (1964)
Lysostaphin disrupts Staphylococcus aureus and Staphylococcus epidermidis biofilms on artificial surfaces
Wu J A, et al.
Antimicrobial Agents and Chemotherapy, 47(11), 3407-3414 (2003)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service