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SRP0103

Sigma-Aldrich

HDAC2 FLAG tag human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

Synonym(s):

HD2, Histone deacetylase 2, Transcriptional regulator homolog RPD3, YAF1, YY1-associated factor 1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥50% (SDS-PAGE)

form

aqueous solution

mol wt

60 kDa

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

Q92769

UniProt accession no.

Q92769

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HDAC2(3066)

General description

Human HDAC2, GenBank Accession No. Q92769, full length with C-terminal DDDDK tag (FLAG), MW = 60 kDa, expressed in baculovirus expression system.
The HDAC2 (histone deacetylase 2) gene is mapped to human chromosome 6q21 and is ubiquitously expressed.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Histone deacetylase 2 (HDAC2) is known to regulate DNA damage signalling, maintain genomic stability and prevents tumor formation and progression. Hdac2 does not possess a DNA binding domain, it forms multi-component repressor complexes for its interaction with transcription factors. Hdac2 also mediates cell proliferation, differentiation and apoptosis. HDAC2 controls response to chronic inflammation in COPD (chronic obstructive pulmonary disease). It activates Wnt signaling pathway via β-catenin T-cell transcription factor. Deletion of HDAC2 along with HDAC1 leads to anemia and thrombocytopenia.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Histone deacetylase 2 (HDAC2) regulates chromosome segregation and kinetochore function via H4K16 deacetylation during oocyte maturation in mouse.
Ma P and Schultz R M
PLoS Genetics, 9(3), e1003377-e1003377 (2013)
Epigenetic changes in diabetes.
Al-Haddad R, et al.
Neuroscience Letters, 625, 64-69 (2016)
Transcription and beyond: the role of mammalian class I lysine deacetylases.
Moser M A, et al.
Chromosoma, 123(1-2), 67-78 (2014)
CRISPR-mediated HDAC2 disruption identifies two distinct classes of target genes in human cells.
Somanath P, et al.
PLoS ONE, 12(10), e0185627-e0185627 (2017)
HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130.
Goder A, et al.
Nature Communications, 9(1), 764-764 (2018)
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