SRP0230
XIAP Active human
recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)
Synonym(s):
API3, BIRC4, IAP3, Baculoviral IAP repeat-containing protein 4 (BIRC4), E3 ubiquitin-protein ligase XIAP (MIHA), IAP-like protein (ILP1), Inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
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biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥65% (SDS-PAGE)
form
aqueous solution
mol wt
57 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... XIAP(331)
General description
XIAP (X-linked inhibitor of apoptosis) is an anti-apoptotic protein belonging to the inhibitor of apoptosis protein (IAP) family, an evolutionary conserved protein family. XIAP contains three tandem copies of BIR (baculovirusIAP repeat) domain, which is composed of ∼70 residues. IAP family members also contain a RING (really interesting new gene) zinc finger domain in their C-terminals.
Application
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
XIAP (X-linked inhibitor of apoptosis) is the most potent caspase-interacting protein and inhibits both intrinsic and extrinsic pathways of apoptosis. Its up-regulation in tumor cells is linked with resistance to chemotherapy. In inflammatory breast cancer (IBC) this protein is linked with resistance to antibody-dependent cellular cytotoxicity (ADCC) driven by cetuximab and trastuzumab. Embelin, an inhibitor of XIAP, functions as an anti-tumor agent in myeloma cell line (HL-60) by suppressing the expression of XIAP. Elevated expression of XIAP in rectal cancer is associated with chemotherapy resistance.
Unit Definition
One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Certificates of Analysis (COA)
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X-linked inhibitor of apoptosis protein mediates tumor cell resistance to antibody-dependent cellular cytotoxicity.
Evans MK et al
Cell Death & Disease, 7, e2073-e2073 (2016)
A single BIR domain of XIAP sufficient for inhibiting caspases.
Takahashi R et al
The Journal of Biological Chemistry, 273(14), 7787-7790 (1998)
Synergism between NF-kappa B inhibitor, celastrol, and XIAP inhibitor, embelin, in an acute myeloid leukemia cell line, HL-60.
Pazhang Y et al
Journal of Cancer Research and Therapeutics, 12(1), 155-160 (2016)
Jun Zhou et al.
Cancer immunology, immunotherapy : CII, 68(8), 1331-1340 (2019-07-19)
Expression of inhibitors of apoptosis protein (IAP) family members is associated with poor prognosis in cancer patients. Immunity to ML-IAP (livin) and survivin has been well studied in patients with a variety of tumors. XIAP, the most potent inhibitor of
Arun Murali et al.
Cell death & disease, 8(6), e2900-e2900 (2017-07-01)
Rho GTPases control fundamental cellular processes and Cdc42 is a well-studied member of the family that controls filopodia formation and cell migration. Although the regulation of Cdc42 activity by nucleotide binding is well documented, the mechanisms driving its proteostasis are
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