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SRP0699

Sigma-Aldrich

STK9 Protein

recombinant

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in baculovirus infected Sf9 cells

assay

70-95% (SDS-PAGE)

form

frozen liquid

mol wt

~84 kDa

concentration

0.1 μg/μL

accession no.

NM_003159

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... STK9(6792)

Application

Useful for Western blot.

Biochem/physiol Actions

Recombinant human STK9 (CDKL5) (1-498) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. STK9 or CDKL5 is a member of Ser/Thr protein kinase family which has protein kinase activity and is associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). STK9 can control nuclear speckle morphology by regulating the phosphorylation state of splicing regulatory proteins, and may be involved indirectly in pre-mRNA processing, by controlling splicing factor dynamics (1). The mutations in the STK9 is a major cause of infantile spasms and early epileptic seizures in female patients, and of a later intractable seizure disorder (2).

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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