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recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
mol wt
~95-100 kDa
concentration
0.5 μg/μL (0.1 ug/uL each PAD)
accession no.
BC025718
BC049947
NM_013358
NM_016233
NM_207421
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PADI1(29943) , PADI2(11240) , PADI3(51702) , PADI4(23569) , PADI6(353238)
Application
Useful for citrullination and Western blot.
Biochem/physiol Actions
The five full length recombinant PAD proteins (1, 2, 3, 4 and 6) expressed by baculovirus in Sf9 insect cells using N-terminal GST tags. This mixture is ideally suited for in vitro citrullination of protein targets.
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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