SRP3200
GDNF from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
ATF-1, Glial-Derived Neurotrophic Factor
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
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biological source
mouse
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.2 ng/mL
mol wt
15.1 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... GDNF(14573)
Related Categories
General description
GDNF (glial cell line derived neurotrophic factor) is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Application
GDNF from mouse has been used for stimulating primary murine cultures of dorsal root ganglion (DRG) neurons.
GDNF from mouse has been used to study the role of NEDL2 (Nedd4-related E3 ubiquitin ligase-2) in GDNF/Ret signaling.
Biochem/physiol Actions
GDNF (glial cell line derived neurotrophic factor) signals through a multicomponent receptor system, composed of a RET (receptor tyrosine kinase) and one of the four GFR-α (α1-α4) receptors (GDNF family receptors). GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson′s disease monkey model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. It might also be involved in the treatment of non-alcoholic fatty liver disease. GDNF is needed for proper spermatogonial stem cell survival and proliferation.
Sequence
MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Physical form
Lyophilized from 10 mM Sodium Citrate.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Peripheral expression and biological activities of GDNF, a new neurotrophic factor for avian and mammalian peripheral neurons.
Trupp M, et al.
The Journal of Cell Biology, 130, 137-148 (1995)
Functional recovery in parkinsonian monkeys treated with GDNF.
Gash DM, et al.
Nature, 380, 252-255 (1996)
A rapid induction mechanism for Lin28a in trophic responses
Amen Am, et al.
Molecular Cell, 65(3), 490-503 (2017)
Peritubular myoid cells participate in male mouse spermatogonial stem cell maintenance.
Chen LY, et al.
Endocrinology, 155, 4964-4974 (2014)
NEDL2 is an essential regulator of enteric neural development and GDNF/Ret signaling.
Wei R, et al.
Cellular Signalling, 27, 578-586 (2015)
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