G1777
Glial Cell Line-derived Neurotrophic Factor human
recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE)
Synonym(s):
ATF, GDNF
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About This Item
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biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥98% (SDS-PAGE)
form
lyophilized powder
mol wt
~30 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... GDNF(2668)
Related Categories
General description
Glial cell line-derived neurotrophic factor (GDNF) human gene is located on human chromosome 5p13.2.
Application
Glial cell line-derived neurotrophic factor human (GDNF) has been used as a component in the neurobasal medium for neural differentiation. It has also been used for self-renewal, expansion and differentiation of spermatogonial stem cells (SSCs).
Biochem/physiol Actions
Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share ~93% sequence homology, with strong species cross-reactivity. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using the Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.
Glial cell line-derived neurotrophic factor human (GDNF) acts as a morphogen in kidney development and modulates spermatogonial differentiation. Mutations in this gene may be associated with Hirschsprung′s disease, Tourette syndrome (TS) and attention deficit/ hyperactivity disorder (ADHD). It is used to treat Parkinson′s disease.
Physical form
Lyophilized from a 0.2 μm filtered solution of 10 mM sodium citrate and 150 mM sodium chloride containing 0.5 mg bovine serum albumin.
Analysis Note
The biological activity of GDNF is determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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