SRP3267
NT-3 human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym(s):
HGNF, NGF-2, Nerve growth factor-2
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
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biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
20-50 ng/mL ED50
mol wt
27.2 kDa
packaging
pkg of 10 μg
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... NTF3(4908)
Related Categories
General description
NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family. This protein is composed of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.
Biochem/physiol Actions
NT3 functions on two structurally unrelated receptor types- the p75 neurotrophin receptor (p75NTR) and the receptor tyrosine kinase (TRK). It promotes the metastatic invasion of brain by breast cancer cells, by promoting the EMT (epithelial-mesenchymal transition) transition of breast cancer cells and suppressing the immune response in brain. This protein is responsible for the indction and survival of neurite outgrowth from a subset of neural crest (NC) and placode-derived neurons.
Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues).
Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues).
Physical form
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Neurotrophin-3 modulates breast cancer cells and the microenvironment to promote the growth of breast cancer brain metastasis.
Oncogene, 32(35), 4064-4077 (2013)
Neurotrophin 3 is a mitogen for cultured neural crest cells.
Kalcheim C et al
Proceedings of the National Academy of Sciences of the USA, 89(5), 1661-1665 (1992)
Cloning and expression of a cDNA encoding a novel human neurotrophic factor.
Kaisho Y et al
Febs Letters, 266(1-2), 187-191 (1990)
P C Maisonpierre et al.
Genomics, 10(3), 558-568 (1991-07-01)
The development and maintenance of the vertebrate nervous system depends upon neuronal survival proteins known as neurotrophic factors. Nerve growth factor (NGF) remains the best characterized neurotrophic molecule. Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are two recently cloned neurotrophic
Yanjie Li et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(6), 1055-1065 (2015-03-12)
Friedreich's ataxia (FRDA) is an autosomal recessive neurological disease caused by expansions of guanine-adenine-adenine (GAA) repeats in intron 1 of the frataxin (FXN) gene. The expansion results in significantly decreased frataxin expression. We report that human FRDA cells can be
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