SRP5348
GST protein, tag-free
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
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recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
~25 kDa
technique(s)
ligand binding assay: suitable
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
Escherichia coli IAI39 ... gst> gst
gst(7152360)
General description
Glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial and microsomal proteins that are used to create the so-called GST gene fusion system. These proteins are capable of multiple reactions with a multitude of substrates. They have a role in detoxication and toxification mechanisms. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione.
Application
GST protein, tag-free has been used in in vitro F-actin binding assay. It has also been used as a protein tag for TPX2 (targeting protein for Xklp2).
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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