T3568
TrypZean® bovine
recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP)
Synonym(s):
Trypsin bovine
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About This Item
Recommended Products
recombinant
expressed in corn
Quality Level
form
lyophilized powder
specific activity
≥3,350 units/mg solid (USP)
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... TRYP8(282603)
Related Categories
General description
TrypZean® eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
Application
Eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
TrypZean® has been used in a study to assess the use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells. TrypZean® has also been used in a study to compare a recombinant trypsin with the porcine pancreatic extract on sperm used for the in vitro production of bovine embryos.
Biochem/physiol Actions
For dissociation of cultured cells.
Preparation Note
Manufactured by Applied Biotechnology Institute utilizing proprietary transgenic plant protein expression system.
Other Notes
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer
Legal Information
Trypzean is a registered trademark of ProdiGene, Inc.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
wgk_germany
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificates of Analysis (COA)
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Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
Pedro P Carvalho et al.
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Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells
K J Mattson et al.
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The objective was to compare the effects of treating bovine semen with two trypsin products (the porcine pancreas extract and a recombinant) and a control (no trypsin) on in vitro embryo production. Our hypothesis was that the trypsin treatments would
240 recombinant bovine trypsin made in maize inactivates bovine herpes virus-1 adsorbed to the bovine Zona Pellucida
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A simple and efficient method based on magnetic-bead technology has been developed for the separation of phosphorylated and nonphosphorylated low-molecular-weight biomolecules, such as nucleotides, phosphorylated amino acids, or phosphopeptides. The phosphate-binding site on the bead is an alkoxide-bridged dinuclear zinc(II)
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Codon-usage bias has been observed in almost all genomes and is thought to result from selection for efficient and accurate translation of highly expressed genes. Codon usage is also implicated in the control of transcription, splicing and RNA structure. Many
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