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T5327

Sigma-Aldrich

Anti-TTP (N-terminal) antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-G0/G1 switch regulatory protein 24, Anti-GOS24, Anti-Growth factor-inducible nuclear protein NUP475, Anti-NUP475, Anti-RNF162A, Anti-TIS11, Anti-Tristetraprolin, Anti-Zfp36, Zinc finger protein 36 homolog Protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours
western blot: 0.5-1.0 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours

UniProt accession no.

P26651

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ZFP36(7538)
mouse ... Zfp36(22695)
rat ... Zfp36(79426)

Related Categories

General description

TTP belongs to a family of human ARE (AU-rich element found in 3′ UTR mRNA) binding protein TTP mRNA is widely distributed among tissue types, with a higher expression level in spleen, lymph nodes, and thymus.
Tristetraprolin (TTP), also known as zinc-finger protein 36 (ZFP36), is a RNA-binding protein, encoded by the gene mapped to human chromosome 19q13.2. TTP is ubiquitously expressed and is characterized with a highly homologous RNA binding domain (RBD) with two CCCH zinc fingers (ZFs).

Immunogen

synthetic peptide corresponding to amino acids 51-67 of human TTP, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Application

TTP (N-terminal) antibody produced in rabbit has been used in following studies:
  • RNA EMSA supershift
  • immunoblotting
  • ribonucleoprotein immunoprecipitation (RNP-IP)

Biochem/physiol Actions

In activated monocytes and T lymphocytes, it regulates the expression of tumor necrosis factor a (TNF- α) by binding to a conserved AU rich element within the 3′ UTR of TNF-α mRNA. TTP is a phosphoprotein reported to be a substrate for kinases like MAPK activated protein kinase 2 (MAPKAPK2). The p38 signal transduction pathway is required for the expression of TTP protein and mRNA.
Tristetraprolin (TTP) plays a key role in maintaining the stability of certain adenosine/uridine AU-rich element (ARE) mRNAs, by removing of the poly(A) tail and enhancing mRNA turnover. The encoded protein participates in mRNA binding and destabilization.

Target description

TTP (N-terminal) (also known as Tristetraproline, Zfp-36, TIS11A, and Growth factor-inducible nuclear protein NUP475) is an RNA-binding protein that suppresses inflammation by accelerating the degradation of cytokine mRNA. TTP belongs to a family of human

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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A pathogenetic role for TNFalpha in the syndrome of cachexia, arthritis, and autoimmunity resulting from tristetraprolin (TTP) deficiency
Taylor GA, et al.
Immunity, 4(5), 445-454 (1996)
Nal Ae Yoon et al.
Oncotarget, 7(8), 8931-8943 (2016-02-04)
Inhibition of epithelial-mesenchymal transition (EMT)-inducing transcription factors Twist and Snail prevents tumor metastasis but enhances metastatic growth. Here, we report an unexpected role of a tumor suppressor tristetraprolin (TTP) in inhibiting Twist and Snail without enhancing cellular proliferation. TTP bound
ZFP36-FOSB Fusion Defines a Subset of Epithelioid Hemangioma with Atypical Features
Antonescu CR
Genes Chromosomes Cancer, 53, 951-959 (2014)
The tandem CCCH zinc finger protein tristetraprolin and its relevance to cytokine mRNA turnover and arthritis
Carrick DM, et al.
Arthritis Research & Therapy, 6(6), 248-248 (2004)
ELAVL1 Modulates Transcriptome-wide miRNA Binding in Murine Macrophages
Lu YC
Cell Reports, 9, 2330-2343 (2014)
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