ABE285
Anti-Tristetraprolin Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Tristetraprolin, TTP, G0/G1 switch regulatory protein 24, Growth factor-inducible nuclear protein NUP475, Protein TIS11A, TIS11, Zinc finger protein 36 homolog, Zfp-36
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
RIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ZFP36(7538)
General description
Tristetraprolin (TTP, Nup 475, TIS11, G0S24, or ZFP36) is a member of the CCCH tandem zinc finger protein family. It is a nuclear RNA-binding protein that functions as a trans-acting factor. It is involved in post-transcriptional regulation of cytokine genes, most notably Tumor Necrosis Factor alpha (TNF-α). Upon activation by lipopolysaccharides, TTP binds TNF-α mRNA at AU-rich domains in the 3′-untranslated Region (3′-UTR) and induces deadenylation, thereby destabilizing the mRNA and tagging it for degradation. To produce this effect, TTP recruits other trans-acting factors to its C-terminal domain and mRNA-decay enzymes to its N-terminal domain. TTP is deactivated by phosphorylation driven by mitogen-activated protein kinase-activated protein kinase 2 (MK2) and p53. Therefore, TTP/MK2 regulates the biosynthesis of TNF-α and mediates inflammatory responses involving TNF-α. Knockout of TTP in mice produces arthritis, alopecia, dermatitis, among a range of other phenotypes related to overexpression of TNF-α.
Immunogen
KLH-conjugated linear peptide corresponding to human Tristetraprolin.
Application
Anti-Tristetraprolin Antibody is an antibody against Tristetraprolin for use in Western Blotting, ICC, Immunoprecipitation, RNA Binding Protein Immunoprecipitation (RIP).
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Tristetraprolin in human leukocytes. (Brooks, S., et al. (2002). Arthritis and Rheumatism. 46(5):1362–1370.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Quality
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Tristetraprolin on 10 µg of A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Tristetraprolin on 10 µg of A431 cell lysate.
Target description
~40 kDa observed. Uniprot gives a calculated molecular weight of 34 kDa An uncharacterized band appears at ~53 kDa in some lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing PBS without 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A431 cell lysate
A431 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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