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WH0007345M1

Sigma-Aldrich

Monoclonal Anti-UCHL1 antibody produced in mouse

clone 1B8-4D2, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-PARK5, Anti-PGP9.5, Anti-ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1B8-4D2, monoclonal

form

buffered aqueous solution

species reactivity

human, rat, mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
western blot: 1-5 μg/mL

isotype

IgG1κ

GenBank accession no.

BC000332

UniProt accession no.

P09936

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... UCHL1(7345)

General description

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease
Ubiquitin C-terminal hydrolase L1 (UCHL1) is encoded by the gene mapped to human chromosome 4p14. The encoded protein is a deubiquitinase, which is part of the ubiquitin proteasome system (UPS). UCHL1 is specifically expressed in the nervous system and gonads.

Immunogen

UCHL1 (AAH00332, 1 a.a. ~ 223 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA

Biochem/physiol Actions

Ubiquitin C-terminal hydrolase L1 (UCHL1) catalyzes the hydrolysis of carboxy-terminal ubiquitin esters and amides. It plays an essential role in epithelial-mesenchymal transition. In addition, UCHL1 is also implicated in the cell invasion and in the cell cycle regulation. UCHL1 gene acts as an oncogene in various types of cancers including glioma, gastric cardiac adenocarcinoma (GCA) and colorectal cancer. It also acts as a susceptibility gene for Parkinson′s disease.

Physical form

Solution in phosphate buffered saline, pH 7.4

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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UCH-LI acts as a novel prognostic biomarker in gastric cardiac adenocarcinoma
Yang H, et al.
International Journal of Clinical and Experimental Pathology, 8(11), 13957-13957 (2015)
UCHL1 acts as a colorectal cancer oncogene via activation of the beta-catenin/TCF pathway through its deubiquitinating activity
Zhong J, et al.
International Journal of Clinical and Experimental Pathology, 30(2), 430-436 (2012)
UCHL1 is a Parkinson's disease susceptibility gene
Maraganore D, et al.
Annals of Neurology, 55(4), 512-521 (2004)
Ubiquitin carboxyl-terminal esterase L1 (UCHL1) is associated with stem-like cancer cell functions in pediatric high-grade glioma
Sanchez-Diaz PC, et al.
PLoS ONE, 12(5), e0176879-e0176879 (2017)
Daisuke Fujisawa et al.
The Journal of allergy and clinical immunology, 134(3), 622-633 (2014-06-24)
Wheal reactions to intradermally injected neuropeptides, such as substance P (SP) and vasoactive intestinal peptide, are significantly larger and longer lasting in patients with chronic urticaria (CU) than in nonatopic control (NC) subjects. Mas-related gene X2 (MrgX2) has been identified
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