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46401-U

Supelco

rac-β-Tocopherol solution

50 mg/mL in hexane, certified reference material

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About This Item

CAS Number:
148-03-8
UNSPSC Code:
85151701
eCl@ss:
34058016
NACRES:
NA.24

grade

certified reference material

Quality Level

100

CofA

current certificate can be downloaded

packaging

ampule of 1 mL

concentration

50 mg/mL in hexane

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

application(s)

pharmaceutical (small molecule)
vitamins, nutraceuticals, and natural products

format

single component solution

storage temp.

-10 to -25°C

InChI

1S/C28H48O2/c1-20(2)11-8-12-21(3)13-9-14-22(4)15-10-17-28(7)18-16-25-24(6)26(29)19-23(5)27(25)30-28/h19-22,29H,8-18H2,1-7H3/t21?,22?,28-/m1/s1

InChI key

WGVKWNUPNGFDFJ-CBIUGAAKSA-N

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General description

β-Tocopherol is a form of fat-soluble vitamin E and shows anti-oxidant, anti-inflammatory, anti-cell proliferative, and anti-cancer properties.

Application

The analytical standard in solution can also be used as follows:
  • Simultaneous determination of vitamin A and all four tocopherol isomers in human serum samples by ultra-high performance liquid chromatography (UHPLC) combined with fluorescence detection following their liquid-liquid extraction (LLE)
  • Multi-residue analysis of vitamin B (B1, B2, B5, and B9), and vitamin E (α-tocopherol, β-tocopherol, α-tocotrienol, and β-tocotrienol) in grass and grain samples of four different wheat varieties by liquid chromatography electrospray ionization/multistage mass spectrometry (LC-ESI-MS/MS)
  • Separation and estimation of four tocopherols and four tocotrienols in pecan nut cultivar samples using non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) coupled with atmospheric pressure chemical ionization-tandem mass spectrometric (APCI-MS/MS) detection
  • Analysis of different cold pressed oil samples to detect and quantify eight tocol homologs using a chemometrically-assisted optimized high-performance liquid chromatography (HPLC) with fluorescence detection (FLD) based method
  • Development and validation of normal-phase high-performance liquid chromatography (NP-HPLC) method, along with fluorescence detection, to simultaneously determine four tocopherols and four lignans in sesame oil samples

Other Notes

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Asp. Tox. 1 - Flam. Liq. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3

target_organs

Central nervous system, Nervous system

wgk_germany

WGK 3

flash_point_f

closed cup

flash_point_c

closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
LRAD0885
LRAD4524
LRAD0885
LRAD0850
LRAC8991

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(±)−α−Tocopherol (Vitamin E) solution

Rapid determination of lipophilic vitamins in human serum by ultra-high performance liquid chromatography using a fluorinated column and high-throughput miniaturized liquid-liquid extraction
Cervinkova B, et al.
Journal of Separation Science, 40(17), 3375-3382 (2017)
Analysis of the components of pumpkin seed oil in suppositories and the possibility of its use in pharmaceuticals
A.O.V, et al.
Journal of Chemical and Pharmaceutical Research, 6(5), 1106-1116 (2014)
Qualitative and quantitative analysis of retinol, retinyl esters, tocopherols and selected carotenoids out of various internal organs from different species by HPLC
Schaffer MW, et al.
Analytical Methods : Advancing Methods and Applications, 2(9), 1320-1332 (2010)
Pressurized liquid extraction and dispersive liquid-liquid microextraction for determination of tocopherols and tocotrienols in plant foods by liquid chromatography with fluorescence and atmospheric pressure chemical ionization-mass spectrometry detection
Vi?as P, et al.
Talanta, 119(9), 98-104 (2014)
Xiaofeng Fang et al.
Developmental cell, 48(3), 371-382 (2019-01-01)
As integral regulators in plant development and stress response, microRNAs (miRNAs) themselves need to be tightly regulated. Here, we show that tocopherols (vitamin E), lipid-soluble antioxidants synthesized from tyrosine in chloroplasts, positively regulate the biogenesis of miRNAs. Tocopherols are required

Protocols

Determination of Vitamin A and E Using HPLC with UV Detection Following the Chinese National Standard (GB) Method

Following GB method for Vitamin A and Vitamin E as it is written with a 250 x 4.6 mm Ascentis® Express C30 column gives baseline resolution between the critical pair (gamma-, and beta-tocopherol; peaks 3 and 4).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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