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58201C40

Supelco

SUPELCOSIL™ LC-Diol HPLC Column

5 μm particle size, L × I.D. 25 cm × 4 mm

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Quality Level

100

agency

suitable for USP L20

product line

SUPELCOSIL™

feature

endcapped: no

manufacturer/tradename

SUPELCOSIL™

packaging

1 ea of

extent of labeling

3.5% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4 mm

surface area

170 m2/g

surface coverage

surface coverage 3.8 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

diol phase

particle size

5 μm

pore size

120 Å pore size

pH range

2-7.5

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

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General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively shield the silica surface from interacting with proteins. A well-known use of diol columns, under normal phase conditions, is the separation of steroids and sterols.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Description
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Certificates of Analysis (COA)

Lot/Batch Number
281968-01
281615-01
279998-01
279071-01
279005-01

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