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G005748

HPLC Analysis of Tamoxifen E/Z isomers and Related Compounds on Ascentis® Express RP-Amide

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 10 cm x 3 mm I.D., 2.7 μm particles (53918-U)
mobile phase: [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient: held at 25% B for 2 min; 25 to 40% B in 15 min; 40 to 25% B in 0.1 min; held at 25% B for 2.9 min
flow rate: 0.6 mL/min
pressure: 2292 psi (158 bar)
column temp.: 35 °C
detector: UV, 260 nm
injection: 2 μL
sample: 10 mg/L in 10:90, water: methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Tamoxifen

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