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1.01376

Sigma-Aldrich

Neutral red (C.I. 50040)

for microscopy Certistain®

Synonym(s):

Neutral red (C.I. 50040)

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About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
553-24-2
Molecular Weight:
288.78
MDL number:
MFCD00012651
UNSPSC Code:
41116130
EC Index Number:
209-035-8

Quality Level

400

form

solid

composition

Dye, ≥90% spectrophotometric assay

pH

3.1 (20 °C, 10 g/L in H2O)

solubility

50 g/L

bulk density

350‑500 kg/m3

εmax

1420-1610 at 540-543 nm in 50% ethanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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General description

Neutral red (C.I. 50040) - for microscopy Certistain®, is a dry staining dye that is used to prepare a staining solution, that makes target structures in hematological specimen materials evaluable for diagnostic purposes. Neutral red belongs to the group of azines. Neutral red can be used for several staining such as supravital staining of leukocytes .In histology for nuclear staining, fluorescence staining, polychrome staining or for counterstaining in Gram staining.
As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 540 - 543 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; ethanol 50 %): 1420 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 12 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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