Skip to Content
MilliporeSigma
All Photos(4)

Documents

N4638

Sigma-Aldrich

Neutral Red

BioReagent, Powder

Synonym(s):

3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
553-24-2
Molecular Weight:
288.78
Colour Index Number:
50040
Beilstein/REAXYS Number:
3918740
EC Number:
209-035-8
MDL number:
MFCD00012651
UNSPSC Code:
12171500
PubChem Substance ID:
24897707
NACRES:
NA.47

product name

Neutral Red, powder, BioReagent, suitable for cell culture

product line

BioReagent

Quality Level

200

form

powder

composition

Dye content, ≥90%

technique(s)

cell culture | mammalian: suitable
titration: suitable

color

brown to black

pH

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

SMILES string

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.

Application

Neutral red has been used in several histological staining applications that include:
  • as a nuclear stain
  • as a counterstain in the Gram procedure, either alone, or as a component of the Twort stain, a fast green-neutral red mixture.
  • fluorescent staining of nucleic acids in nervous tissue, of lipids and hydrophobic substances such as suberin and of phenolic compounds in algal cell walls
  • detection of viable yeast cells in dandruff samples and of mussel larvae
  • Neutral red has also been used as a stain in flow cytometry.
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Preparation Note

Purified

related product

Product No.
Description
Pricing

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 2

1 of 2

Riboflavin Binding Protein from chicken egg white lyophilized powder

Sigma-Aldrich

R5258

Riboflavin Binding Protein from chicken egg white

Thiazolyl Blue Tetrazolium Bromide powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

Sigma-Aldrich

M5655

Thiazolyl Blue Tetrazolium Bromide

Pieter C Vogelaar et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 33(12), 2128-2138 (2018-04-17)
Mitochondrial dysfunction plays an important role in kidney damage in various pathologies, including acute and chronic kidney injury and diabetic nephropathy. In addition to the well-studied ischaemia/reperfusion (I/R) injury, hypothermia/rewarming (H/R) also inflicts acute kidney injury. Substituted 6-hydroxychromanols are a
K R Sindhu et al.
Acta biomaterialia, 115, 197-209 (2020-08-20)
Hydrogels used in regenerative medicine are often designed to allow cellular infiltration, degradation, and neovascularization. Low molecular weight hydrogels (LMWHs), formed by self-assembly via non-covalent interactions, are gaining significant interest because they are soft, easy to use and injectable. We
Valerio Matozzo et al.
Marine environmental research, 79, 116-121 (2012-06-26)
The effects of ibuprofen (IBU) on the immune parameters of the clam Ruditapes philippinarum were investigated after a 7-day exposure to sublethal IBU concentrations (0, 0 + ethanol, 100, 500, and 1000 μg/L). Total haemocyte count (THC), haemocyte diameter and
I Crooks et al.
Regulatory toxicology and pharmacology : RTP, 65(2), 196-200 (2012-12-12)
Particulate matter (PM) collected from mainstream tobacco smoke is a test article commonly used for in vitro genotoxicity and cytotoxicity testing of combustible tobacco products. However, little published data exists concerning the stability of PM. We completed a 2 year
Kun Guo et al.
Biosensors & bioelectronics, 47, 184-189 (2013-04-13)
This study introduces a novel and simple method to covalently graft neutral red (NR) onto carbon surfaces based on spontaneous reduction of in situ generated NR diazonium salts. Immobilization of neutral red on carbon surface was achieved by immersing carbon
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service