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861251

Sigma-Aldrich

Neutral Red

certified by the Biological Stain Commission

Synonym(s):

3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red

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About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
553-24-2
Molecular Weight:
288.78
Colour Index Number:
50040
Beilstein/REAXYS Number:
3918740
EC Number:
209-035-8
MDL number:
MFCD00012651
UNSPSC Code:
12171500
PubChem Substance ID:
24888533
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

100

form

powder

composition

Dye content, 50-60%

technique(s)

titration: suitable

pH

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

solubility

water: 1 mg/mL

λmax

540 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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Application

Neutral red has been used as a staining agent for micro- and meso-plankton. It has been used as a staining agent for nematocytes.
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Biochem/physiol Actions

Neutral red is useful as an indicator for preparing neutral red paper, and as a biological stain. Neutral dye has low toxicity and is a vital dye. It can also be used as a pH indicator. It is used for cell viability assay. The dye accumulates in the lysosomes of living healthy cells, but not in damaged cells.

Suitability

Certified for use in the method of Doan & Ralph for the supravital staining of living blood cells.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
11714LE
11814LE
06601HV
09302CE
07607TD

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Clung,C.E., Ed.
Handbook of Microscopical Technique, 571-571 (1950)
Catostylus tagi: partial rDNA sequencing and characterisation of nematocyte structures using two improvements in jellyfish sample preparation.
Parracho T and Morais Z
Journal of Venomous Animals and Toxins including Tropical Diseases, 21, 40-40 (2015)
The Anatomical Record, 27, 241-241 (1924)
PH and Brain Function (1998)
Dead or alive? Viability assessment of micro-and mesoplankton.
Zetsche EV and Meysman FJ
Journal of Plankton Research, 34, 493-509 (2012)
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