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487923

Sigma-Aldrich

Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3)

liquid, clone CC22.8C7.3, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

CC22.8C7.3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)

General description

Purified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
Recognizes nitrotyrosine.
This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.

Immunogen

peroxynitrite-treated KLH

Application

Immunoblotting (2 µg/ml)

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.

Reconstitution

Reconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Mouse macrophages treated with peroxynitrite

Other Notes

Myatt, L., et al. 1996. Hypertension28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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