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MABN770

Sigma-Aldrich

Anti-Melanopsin Antibody, clone 1F8.1

clone 1F8.1, from mouse

Synonym(s):

Melanopsin, Opsin-4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1F8.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aλ

NCBI accession no.

NP_150598

UniProt accession no.

Q9UHM6

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... OPN4(94233)

Related Categories

General description

Melanopsin (UniProt Q9UHM6; also known as Opsin-4) is encoded by the OPN4 (also known as MOP) gene (Gene ID 94233) in human. Opsins belong to the superfamily of G protein-coupled receptors (GPCRs). Melanopsin is a blue light-sensitive opsin protein that is capable of rendering cells intrinsically light responsive. Melanopsin expression defines a population of retinal ganglion cells that are intrinsically photosensitive (ipRGCs). Melanopsin allows mammals to measure irradiance for centrally mediated responses such as circadian entrainment. In addition, the information generated by melanopsin is used by the visual system as a signal for light adaptation. Using melanopsin-dependent phototransduction mechanism coupled with extrinsic signals originating with rods and cones, ipRGCs can encode light levels over many decimal orders, allowing the visual system to adapt to a broad range of light intensity from starlight to broad daylight.

Specificity

Clone 1F8.1 is expected to react with both spliced isoforms of human melanopsin.

Immunogen

GST-tagged recombinant protein corresponding to human Melanopsin C-terminal sequence

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Melanopsin in human retina tissue.
This Anti-Melanopsin Antibody, clone 1F8.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Melanopsin .

Quality

Evaluated by Western Blotting in human retinal pigment epithelia (RPE) ARPE-19 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Melanopsin in 10 µg of human retinal pigment epithelia (RPE) ARPE-19 cell lysate.

Target description

~52 kDa observed

Physical form

Format: Purified
Purified mouse monoclonal IgG2aλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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