SRP0263
PDE4B1 Active human
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonym(s):
DPDE4, PDE4B, Phosphodiesterase 4B, cAMP-specific, Phosphodiesterase 4B1
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥70% (SDS-PAGE)
form
aqueous solution
specific activity
≥10000 units/μg
mol wt
109 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDE4B(24626)
General description
Human PDE4B1 (GenBank Accession No. NM_001037341), full length with N-terminal GST tag, MW = 109 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Unit Definition
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 30°C
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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