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HomePEPscreen®: Custom Peptide Libraries

PEPscreen®: Custom Peptide Libraries

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Important Update

March 22, 2022: Please be advised of a recent strategic business decision in which we will be discontinuing our PEPscreen® Custom Peptide Library product offering. This notice provides several months for ordering preparation as well as validating an alternative supplier. Globally, the last order intake date will be September 30, 2022. It is our intention that by providing a 6-month discontinuation notice, you will have ample time to plan and establish business continuity. It has been a pleasure supplying you with this product.

Experience affordable and fast delivery of custom peptide libraries for target identification. The PEPscreen peptide library is configured in a convenient plate format, perfect for screening applications, epitope mapping and peptide microarray production.

Product Specifications

  • Sequence Length: 6 to 20 amino acids
  • Amount: 0.5-2 mg or 2-5 mg
  • Modifications: Comprehensive offering including phosphorylation, biotin, fluorescein, stable isotopes, etc.
  • Format: Supplied dry in a 96-well tube rack
  • Options: Normalization and aliquoting (stock and copy libraries)
  • Minimum library size of 24 peptides

Peptide Library Design

We offer complimentary peptide library design services for overlapping peptide fragment libraries, alanine scanning libraries, truncation libraries and positional scanning libraries. A peptide design tool for generating different types of peptide libraries is currently in development. To request a quote or help designing a custom peptide library, please complete the online form.

Overlapping Peptide Library – Overlapping peptide fragments to cover the full length of your protein of interest.

Alanine Scanning Library – Systematically substitute each amino acid position on your peptide sequences with alanine- the smallest of the naturally occurring amino acids that maintains it chirality. Alanine mutagenesis can be a powerful tool for probing the effect of individual amino acids side chain to protein function and properties.

Truncation Library – Systematically remove flanking amino acids to determine the minimum length required for optimum activity. Once the essential amino acid residues for protein activity are identified by alanine scanning, the direction of truncation can be selected around these residues, rather than removing residues from both ends of the peptide sequence.

Positional Scanning Library – Optimize the peptide lead sequence. A selected position on the lead peptide is replaced with different amino acid (natural or unnatural) to improve the peptide properties such as biological activity, enzyme stability and solubility. The substitution can be performed on a single or multiple positions on a peptide sequence. A peptide library constructed using positional scanning can be very informative in studying protein interfaces whether in epitope mapping or protein-protein interaction.

Need help with your design?

Publications using PEPscreen® peptide libraries

1.
Planque SA, Mitsuda Y, Nishiyama Y, Karle S, Boivin S, Salas M, Morris M, Hara M, Liao G, Massey RJ, et al. 2012. Antibodies to a Superantigenic Glycoprotein 120 Epitope as the Basis for Developing an HIV Vaccine. J.I.. 189(11):5367-5381. https://doi.org/10.4049/jimmunol.1200981
2.
Cwirla SE. 1997. Peptide Agonist of the Thrombopoietin Receptor as Potent as the Natural Cytokine. 276(5319):1696-1699. https://doi.org/10.1126/science.276.5319.1696
3.
Kasetty G, Papareddy P, Kalle M, Rydengård V, Mörgelin M, Albiger B, Malmsten M, Schmidtchen A. 2011. Structure-Activity Studies and Therapeutic Potential of Host Defense Peptides of Human Thrombin. Antimicrob. Agents Chemother.. 55(6):2880-2890. https://doi.org/10.1128/aac.01515-10
4.
Kemp BP, Doughty J. 2007. S cysteine-rich (SCR) binding domain analysis of the Brassica self-incompatibility S-locus receptor kinase. New Phytol. 175(4):619-629. https://doi.org/10.1111/j.1469-8137.2007.02126.x
5.
Hvidsten TR, Kryshtafovych A, Fidelis K. 2009. Local descriptors of protein structure: A systematic analysis of the sequence-structure relationship in proteins using short- and long-range interactions. Proteins. 75(4):870-884. https://doi.org/10.1002/prot.22296
6.
Beissbarth T, Tye-Din JA, Smyth GK, Speed TP, Anderson RP. 2005. A systematic approach for comprehensive T-cell epitope discovery using peptide libraries. Bioinformatics. 21(Suppl 1):i29-i37. https://doi.org/10.1093/bioinformatics/bti1013
7.
Schilling O, Overall CM. 2008. Proteome-derived, database-searchable peptide libraries for identifying protease cleavage sites. Nat Biotechnol. 26(6):685-694. https://doi.org/10.1038/nbt1408
8.
Kim M, Shin D, Kim J, Lee Y. 2010. Substrate screening of protein kinases: Detection methods and combinatorial peptide libraries. Biopolymers. 94(6):753-762. https://doi.org/10.1002/bip.21506
9.
Oreshkova N, Cornelissen L, de Haan C, Moormann R, Kortekaas J. 2014. Evaluation of nonspreading Rift Valley fever virus as a vaccine vector using influenza virus hemagglutinin as a model antigen. Vaccine. 32(41):5323-5329. https://doi.org/10.1016/j.vaccine.2014.07.051
10.
Mabe S, Nagamune T, Kawahara M. 2015. Detecting protein?protein interactions based on kinase-mediated growth induction of mammalian cells. Sci Rep. 4(1): https://doi.org/10.1038/srep06127
11.
Wrighton NC, Farrell FX, Chang R, Kashyap AK, Barbone FP, Mulcahy LS, Johnson DL, Barrett RW, Jolliffe LK, Dower WJ. 1996. Small Peptides as Potent Mimetics of the Protein Hormone Erythropoietin. Science. 273(5274):458-463. https://doi.org/10.1126/science.273.5274.458
12.
Kong D, Liu J, Liu, Chu, Wang, Duan, Feng, Wang, Yang. 2010. Novel peptide–dendrimer conjugates as drug carriers for targeting nonsmall cell lung cancer. Int J Nanomedicine.(6):59. https://doi.org/10.2147/ijn.s14601
13.
Garsky VM, Lumma PK, Feng D, Wai J, Ramjit HG, Sardana MK, Oliff A, Jones RE, DeFeo-Jones D, Freidinger RM. 2001. The Synthesis of a Prodrug of Doxorubicin Designed to Provide Reduced Systemic Toxicity and Greater Target Efficacy. J. Med. Chem.. 44(24):4216-4224. https://doi.org/10.1021/jm0101996
14.
Wong D, Robertson G. 2004. Applying Combinatorial Chemistry and Biology to Food Research. J. Agric. Food Chem.. 52(24):7187-7198. https://doi.org/10.1021/jf040140i
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